FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23937989
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC413350.17267532372915703TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT69600.029.74660942
ACGTCTG84800.028.59501615
CGTCTGA86350.028.1598816
TCGTATG72500.028.09143843
CACACGT97150.025.33076712
ATCGGAA100800.024.661272
CGTATGC81800.024.5128244
CACGTCT100950.024.24320414
TCGGAAG102950.024.2109953
ACACGTC108350.022.71202513
CAGTCAC110700.021.84022727
GATCGGA116400.021.5528871
TCTCGTA100150.021.23128341
AGTCACC116100.020.97951928
CGGAAGA121950.020.68024
GTATGCC105350.019.46044345
CCAGTCA127200.019.07729126
GCACACG133400.018.64975411
TCCAGTC134600.018.27672825
GTCTGAA135600.018.2307917
Produced by FastQC (version 0.11.5)