FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29429469
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC841380.2858971053810043TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG128900.035.3379215
AGTCACG127350.035.2834328
CTCGTAT120150.034.35052542
CGTCTGA133700.034.22066516
TCGTATG122700.033.30673643
GTCACGC140300.032.17109729
TATCTCG135350.031.94778439
ATCGGAA148250.031.1088092
CACACGT149600.030.79449812
TCGGAAG150150.030.7287313
CGTATGC135100.030.0337344
CACGTCT153350.029.95303514
CGCCAAT152950.029.08033233
ACGCCAA154950.028.8230132
CACGCCA157250.028.6173331
CAGTCAC159750.028.46555727
ACACGTC165850.028.1159513
TCTCGTA152850.027.4681141
TCACGCC170900.026.63445930
GCCAATA169750.026.29955334
Produced by FastQC (version 0.11.5)