FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28429607
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC624220.21956687617947024TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG96350.033.0815315
CTCGTAT92350.032.45841642
TCGTATG94550.032.13204643
CGTCTGA100050.031.7232316
CGTATGC105500.028.8831544
ATCGGAA114350.028.4088882
TCGGAAG115250.028.3806933
GTCACAC110550.028.20345729
CACACGT118500.027.39195612
CACGTCT120650.026.7914914
CAGTCAC125900.025.12244427
ACACGTC133350.024.52668613
AGTCACA128500.024.47397828
AGTGATC125050.024.19539836
TCTCGTA125500.024.09618441
CACAGTG129750.023.87132533
ACACAGT134250.023.22337732
GTATGCC138400.022.40739845
GATCGGA146600.022.393881
CGGAAGA149050.022.202374
Produced by FastQC (version 0.11.5)