FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28460088
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC477470.16776827956399853TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG86600.032.46822715
CGTCTGA89600.031.35601816
CTCGTAT75650.030.93773542
TCGTATG80250.029.27688843
AGTCACT100450.027.2291928
ATCGGAA104500.027.1043662
TCGGAAG105950.026.8167573
CACGTCT106700.026.41515214
CGTATGC87050.026.39565844
CACACGT111500.025.62144912
ACTGACC110200.025.10509932
CAGTCAC114550.024.42780527
ACACGTC124200.023.14598713
GTCACTG120350.022.9886929
AATCTCG113800.022.85375639
TCTCGTA108900.021.83918641
CACTGAC127300.021.71581331
GATCGGA132250.021.5909271
CCAGTCA133650.020.78419726
TGACCAA135400.020.55709534
Produced by FastQC (version 0.11.5)