FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28649731
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC516980.1804484656417891TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG87850.033.03145615
ACTTAGG86500.033.02293432
CGTCTGA89450.032.49091316
CTCGTAT83200.030.5917342
TCGTATG81900.030.47328243
TCACTTA97350.029.25068930
TCGGAAG104700.028.4689663
ATCGGAA103800.028.4572282
AGTCACT100050.028.43926428
TTAGGCA101650.028.06789834
CTTAGGC102550.027.80880233
GTCACTT103950.027.52386529
CGTATGC91550.027.33521844
CACTTAG105050.027.27822731
CACACGT111300.026.51686712
CACGTCT111300.026.47616814
CAGTCAC116250.024.7472127
ACACGTC127700.023.62205313
TAGGCAT122350.023.18494235
TCTCGTA114700.022.73636841
Produced by FastQC (version 0.11.5)