FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29487747
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG54850.025.22131515
CGTCTGA55850.024.68917316
TCGTATG53200.023.94382743
CTCGTAT53400.023.89613242
CGTATGC63100.019.93799844
ATCGGAA72150.019.5502682
TCGGAAG73700.019.2296683
ACCGATG73500.018.86543732
CACGTCT76200.018.47943314
TATCTCG73650.018.26797939
CACCGAT76900.017.94406731
CACACGT81250.017.49733212
GATGTAT78400.017.41659535
CAGTCAC84000.016.93650627
TCTCGTA84250.015.43725541
ACACGTC94000.015.41087313
AGTCACC94800.015.19699228
GTATCTC91550.015.11333838
GATCGGA100250.014.29721
TGTATCT102500.013.497395537
Produced by FastQC (version 0.11.5)