FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24681264
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC362050.1466902181346952TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG66500.031.15318115
CGTCTGA67200.030.92908516
TCGTATG66500.028.96867243
CTCGTAT64200.028.53386342
CGTATGC70800.027.0826844
TCGGAAG82700.025.4882893
ATCGGAA82100.025.4287282
CACGTCT84050.024.96941214
CACACGT84250.024.72364412
CAGTCAC91550.022.3270227
GTCACAT95950.021.35008829
ACACGTC99950.021.06480413
TCACGAT97550.021.03058435
AGTCACA99100.020.71688528
TCTCGTA91250.020.56558241
GATCGGA106300.019.7071291
AGCACAC110050.019.48080310
GAGCACA113350.019.0528079
CGGAAGA113850.018.9307274
CCAGTCA108600.018.82057426
Produced by FastQC (version 0.11.5)