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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-10, 16:04 based on data in: /beegfs/mk5636/logs/html/CBY82ACXX/1

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CBY82ACXX_l01_n01_P1S1
        69.6%
        52%
        30.5
        CBY82ACXX_l01_n01_P1S2
        70.3%
        52%
        30.1
        CBY82ACXX_l01_n01_P1S3
        68.8%
        52%
        31.4
        CBY82ACXX_l01_n01_P1S4
        69.7%
        52%
        28.4
        CBY82ACXX_l01_n01_P1S5
        69.5%
        52%
        32.1
        CBY82ACXX_l01_n01_P1S6
        68.0%
        52%
        29.1
        CBY82ACXX_l01_n01_P1S7
        65.4%
        50%
        26.9
        CBY82ACXX_l01_n01_P1S8
        67.9%
        52%
        27.7
        CBY82ACXX_l01_n01_P1S9
        68.1%
        52%
        26.1
        CBY82ACXX_l01_n01_undetermined
        89.4%
        45%
        16.8
        CBY82ACXX_l01_n02_P1S1
        71.5%
        54%
        30.5
        CBY82ACXX_l01_n02_P1S2
        71.5%
        54%
        30.1
        CBY82ACXX_l01_n02_P1S3
        70.2%
        54%
        31.4
        CBY82ACXX_l01_n02_P1S4
        71.4%
        54%
        28.4
        CBY82ACXX_l01_n02_P1S5
        71.1%
        54%
        32.1
        CBY82ACXX_l01_n02_P1S6
        69.6%
        54%
        29.1
        CBY82ACXX_l01_n02_P1S7
        66.2%
        53%
        26.9
        CBY82ACXX_l01_n02_P1S8
        69.4%
        54%
        27.7
        CBY82ACXX_l01_n02_P1S9
        69.6%
        53%
        26.1
        CBY82ACXX_l01_n02_undetermined
        88.0%
        46%
        16.8

        Lane 1 Demultiplexing Report

        Lane 1 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        16,766,587
        6.0
        P1S1
        30,548,189
        10.9
        P1S2
        30,091,842
        10.8
        P1S3
        31,371,773
        11.2
        P1S4
        28,392,931
        10.2
        P1S5
        32,102,238
        11.5
        P1S6
        29,093,884
        10.4
        P1S7
        26,886,200
        9.6
        P1S8
        27,691,420
        9.9
        P1S9
        26,102,266
        9.4

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CCCCCC
        115942.0
        0.7
        CGCCCC
        92604.0
        0.6
        CGGCCC
        81458.0
        0.5
        CACCCC
        78990.0
        0.5
        TAGGCA
        77420.0
        0.5
        CGCCCA
        75227.0
        0.5
        CTCCCC
        74811.0
        0.5
        CCGCCC
        72481.0
        0.4
        CAGCCC
        70725.0
        0.4
        CCCCCA
        70364.0
        0.4
        GCCCAA
        63089.0
        0.4
        GATGTA
        61464.0
        0.4
        CCCCCT
        59333.0
        0.3
        CGGCCA
        57037.0
        0.3
        TGCCCC
        56706.0
        0.3
        CCGCTC
        55191.0
        0.3
        ATACGA
        53153.0
        0.3
        GCCCCT
        51050.0
        0.3
        ACTGAA
        50263.0
        0.3
        ATCAGA
        48317.0
        0.3

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        1.0
        301,040,768
        279,047,330
        6.0
        6.1

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.