FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16766587
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN653750.38991238944455425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT7600.024.120882
TTCCCTA5750.016.4588188
TCCCTAC6000.014.6309989
TCGGAAG11300.014.6117673
GAGTGTA3100.014.51227928
TACACGA5650.014.33024513
ATCGGAA12050.013.8984522
CGGAAGA12950.013.4401134
AGTGTAG3350.013.42927429
TCGTGTA5300.012.30613514
GTAGAAC4600.012.22292811
GTAGATC4050.012.21879732
GATCGGA14650.012.2093021
TTTCCCT9600.011.9771577
TCAGGTA4950.011.8419437
TAGAACA4950.011.81320412
GTGTAGG5550.011.75025516
AGCGGTT8550.011.57323710
CGGGAAT2757.428389E-811.5264811
CCCTACA7900.011.38678710
Produced by FastQC (version 0.11.5)