FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26102266
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN487260.18667344819794573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22900.012.7382022
GCGTGAC31900.010.140761
GTACTCG53600.09.8171354
GAATCGA37650.09.4773244
ACTTTCG30100.09.18881719
GTAGAAC36200.09.00819911
TACTCGA58800.08.9032945
AGTACTC65050.08.7198063
CTCGAAT28300.08.712951
GGAAAAT111350.08.6342341
GCCCTAT35950.08.57003710
AAAATCC82100.08.5054193
ATAGGGG33150.08.41085134
TAACGAG30550.08.24402818
GTGACTC39600.08.16058353
TCAGGTA46250.07.89037667
GAATTGA76100.07.86420544
TCCCTTA32450.07.831452413
CCCTTAA32400.07.774125614
AATCCGC36900.07.5829015
Produced by FastQC (version 0.11.5)