FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27691420
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22200.010.6945442
GTAGAAC43950.010.54109711
TCAGGTA51650.09.943947
GAATCGA49300.09.3910194
GGAAAAT123300.08.8977181
CAGGTAG55650.08.7786048
GTACTCG51600.08.7535874
CTCGAAT28400.08.6817671
TATAAGG27450.08.4674455
GGTAGAA56400.08.37337110
AAAATCC88100.08.362013
TACTCGA55000.08.2879315
GCGTGAC33900.08.0739431
TAGAACA56850.07.872328312
AGTACTC63050.07.8492473
GAATTGA71350.07.78658254
AATCGAA58150.07.41209755
AATCGAG45250.07.23114165
AGGTAGA67650.07.21723469
GAAAATC101700.07.13690662
Produced by FastQC (version 0.11.5)