FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26886200
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43800.010.98786811
TCAGGTA52150.09.5463367
CTCGAAT29400.09.1562041
CAGGTAG54400.08.9805228
GTACTCG44700.08.4379594
AAAAATC90000.08.4170212
TAGAACA58500.08.26528712
GGTAGAA60250.08.17426410
AAAATCC78450.08.0948513
GAATCGA49550.07.97669274
CGTAGGT15350.07.909631335
GGAAAAT110850.07.6526421
GAATTGA77950.07.44638444
AGATCAG108700.07.29299644
TACTCGA50850.07.23415045
AATTGAC48100.07.1785595
AATTCAC43900.07.1456415
AGGTAGA69750.07.0966219
CGAAAAC44150.07.07072971
AACGTAA9601.8189894E-127.0524645
Produced by FastQC (version 0.11.5)