FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29093884
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47000.011.86675711
TCAGGTA56050.010.93149957
CGTGACT27100.010.6805582
GGTAGAA60900.09.41636310
CAGGTAG62550.09.2855628
CTCGAAT31000.09.1215221
TAGAACA61850.09.09038512
GCGTGAC37300.08.9151261
GGAAAAT115850.08.7478431
GAATCGA50650.08.6504884
GTACTCG50450.08.4609454
AGATCAG105000.08.3456614
ACGGTAC19000.08.1660720
AATCGAA58000.08.0536985
AAAATCC83550.08.03381253
ATATCCA72500.07.81905034
AGAACGG195150.07.6781513
AAAAATC85800.07.59029252
GTGACTC45200.07.5500563
GAATTGA78400.07.54750734
Produced by FastQC (version 0.11.5)