FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32102238
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29100.013.5978182
GTAGAAC44900.011.82091411
GTACTCG66750.010.3192224
TCAGGTA56650.09.980577
TACTCGA67600.09.847265
GCGTGAC43100.09.5517761
GAATCGA53900.09.2598074
AGTACTC78900.09.1362593
AAAATCC98600.08.8922073
TAGAACA60650.08.82542412
CAGGTAG63100.08.7763678
GGAAAAT133800.08.70644951
GGTAGAA61500.08.66644510
GTGACTC50450.08.5103453
AATTGAC54750.08.16058355
GAATTGA84050.08.087744
CTCGAAT33600.08.0785231
TCCGTTA39550.08.01668619
AATCGAA64650.07.88823845
AATTCAC48300.07.80206355
Produced by FastQC (version 0.11.5)