FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28392931
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28550.014.7308492
GCGTGAC39150.011.9025731
GTAGAAC43550.011.67090511
TCAGGTA53200.010.2890187
GTACTCG67950.010.0367564
TACTCGA68850.09.7010635
GAATCGA44100.09.6270924
CTCGAAT31100.09.6010541
AGTACTC77250.09.1261713
GTGACTC49100.09.0660323
TAGAACA57650.09.05052412
GGTAGAA59600.08.86715710
GGAAAAT114500.08.6729011
CAGGTAG59500.08.5886298
AATCGAA55500.08.3347475
CTAGCAC53000.08.31697214
GAATTGA76350.08.1339134
GCTCTAG56950.08.05602311
ATTGACC46250.07.99206356
AATTGAC49500.07.8862855
Produced by FastQC (version 0.11.5)