FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31371773
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32050.015.1687322
GCGTGAC44450.012.0097551
GTAGAAC39200.010.2693911
GTACTCG75000.09.967124
GTGACTC54250.09.8723563
TACTCGA75850.09.6986885
CTAGCAC58400.09.08822214
GGAAAAT129250.08.9080191
GCTCTAG63500.08.88950711
AGTACTC89300.08.5280683
TGACTCT72000.08.186744
GAATTGA84400.08.1613194
AAAATCC98500.07.9609613
AATCGAA63150.07.89714055
CACTCGT62750.07.88287435
TCAGGTA53050.07.8132967
AAAAATC95550.07.71481942
AGAACGG180900.07.6576253
ACTCGTG64450.07.63899836
GAAAAAT175650.07.4691941
Produced by FastQC (version 0.11.5)