FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30091842
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT30650.015.5675722
GCGTGAC42400.012.00486851
GTAGAAC41250.010.6858611
CTCGAAT34600.010.3305091
GTGACTC52450.09.953313
GTACTCG65950.09.6920234
GAATCGA51750.09.2963424
TCAGGTA51100.09.2573957
GAATTGA79150.09.2171034
AATCGAA63650.09.1117655
AATTGAC53900.09.0434225
TACTCGA70200.08.9366655
GGAAAAT141100.08.9143261
AAAATCC96050.08.7763213
AGTACTC78100.08.767843
GCTCTAG62350.08.620625511
CTAGCAC57900.08.50635614
ATTGACC50400.08.2734696
TATAAGG32450.08.1365255
GGTAGAA57200.08.09903510
Produced by FastQC (version 0.11.5)