FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n02_P1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n02_P1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30548189
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29550.014.1562892
GCGTGAC40900.011.5038641
GTAGAAC40050.09.93912711
GGAAAAT132250.09.8008261
GTACTCG63650.09.2606314
GTGACTC50850.09.24393
AAAATCC96200.09.1381313
GAATCGA51250.08.7250874
GAATTGA81800.08.6691154
TACTCGA67250.08.4560755
TCAGGTA52300.08.3131197
AGTACTC74500.08.2507893
GCTCTAG58450.08.19544611
CTAGCAC56100.08.01768414
AATTGAC52950.07.8417395
CTCGAAT31600.07.80266861
AATCGAA66700.07.78148655
AAATCCT102550.07.6857854
AGAACGG177500.07.61352353
GAAAATC111750.07.5069162
Produced by FastQC (version 0.11.5)