FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16766587
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG12450.036.2822315
CGTCTGA12550.035.8140616
CACGTCT14350.031.16453614
CACACGT14450.030.78544612
ACACGTC15250.029.76740813
TCGGAAG18300.027.7640423
ATCGGAA18550.027.2699722
CAGTCAC12350.026.67395427
CCAGTCA13800.024.8516726
GATCGGA21150.024.2629641
CGGAAGA21850.023.6662944
GCACACG18900.023.53758211
AGCACAC19250.023.46131110
CTCCAGT20400.019.68311124
AACTCCA23000.018.69592322
AGTCACT4900.014.73517828
GAACTCC30150.014.6120521
CTGAACT32500.013.8990119
AGAGCAC32600.013.7848468
AGCGGTT5650.011.930531510
Produced by FastQC (version 0.11.5)