FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26102266
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC390880.1497494508714301TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG71200.031.08855415
CGTCTGA72700.030.29240416
AGTCACG70900.030.0043328
CTCGTAT61350.029.52143542
TCGTATG65250.028.03460943
CGTATGC68550.026.2257544
ATCGGAA85900.025.980932
TCGGAAG86400.025.9342373
GTCACGA84000.025.61987129
CACACGT89650.025.09213612
CACGTCT91150.024.6792114
CAGTCAC95900.022.69897327
ACACGTC100950.022.26112613
TCTCGTA93100.019.91072741
GATCGGA115750.019.47921
AGCACAC120200.018.99719210
CGGAAGA119800.018.986564
ACGATCA116950.018.67102432
CCAGTCA115850.018.6343526
TCACGAT116150.018.47018430
Produced by FastQC (version 0.11.5)