FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27691420
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC328250.11853852204040097TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG119850.033.35312715
CGTCTGA121650.032.71168516
TCGGAAG134750.030.1016453
ATCGGAA135800.029.7705762
CACACGT136800.029.49999612
GTCACAC132650.029.35847529
CACGTCT143000.028.06381814
CTCGTAT66500.028.01399642
CAGTCAC147600.026.50684727
ACACGTC156050.026.09172213
CACACTT150450.026.01968831
AGTCACA151450.025.75870128
TCGTATG69850.025.28762643
GATCGGA165850.024.5721021
CCAGTCA161000.024.27224326
TCACACT164650.023.91237830
CGGAAGA172350.023.7839454
ACACTTG164950.023.74593432
TCCAGTC170100.023.30317925
GAGCACA177150.023.1763469
Produced by FastQC (version 0.11.5)