FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26886200
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC445260.16560912289576066TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG104350.032.65881715
CGTCTGA107950.031.56968316
CTCGTAT79250.029.63836142
ATCGGAA120350.028.974932
CACGTCT118800.028.80003514
TCGGAAG120950.028.7373333
CACACGT119900.028.6296212
TCGTATG81650.027.50165243
ACACGTC132950.025.98861313
GATCGGA141500.024.8404771
CAGTCAC135300.024.75783227
CGGAAGA145750.024.1887024
CGTATGC90400.023.869244
AGTCACC144550.023.48500828
CCAGTCA147700.022.63321126
TCTCGTA108300.021.99726941
TCCAGTC155850.021.62147325
GCACACG162400.021.44259311
GAGCACA170450.020.8935689
ACTCCAG168050.020.56842223
Produced by FastQC (version 0.11.5)