FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29093884
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC421800.14497892409277496TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG83400.030.53246515
AGTCACG84100.029.84608328
CGTCTGA87650.029.1289916
CTCGTAT71650.028.48032642
TCGTATG73550.026.46157343
GTCACGC97250.025.9492329
TCGGAAG101250.025.663123
ATCGGAA103550.024.9849032
TATCTCG92850.024.49416539
CACGTCT106050.024.37210514
CACACGT107100.024.30119112
CACGCCA111700.022.31005731
CGTATGC88200.022.01555644
ACGCCAA112850.021.84325232
ACACGTC120750.021.8149113
TCACGCC117700.021.72741730
CAGTCAC117300.021.68647227
CGCCAAT114400.021.56638133
CCAGTCA127600.019.84709226
GATCGGA131950.019.8337841
Produced by FastQC (version 0.11.5)