FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32102238
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC562130.17510617172547285TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG92700.032.0809115
CGTCTGA93550.031.50086216
CTCGTAT88600.030.77837642
TCGTATG92750.030.03447743
TCGGAAG109150.027.7426173
ATCGGAA110200.027.5196672
GTCACAC107200.027.25529929
CGTATGC102300.026.98897244
CACACGT113750.026.34206412
CACGTCT115600.025.9788814
CAGTCAC123650.023.97522527
ACACGTC128250.023.57430513
AGTCACA124700.023.53868328
ACACAGT134550.021.6146332
AGTGATC129900.021.5437436
CACAGTG134550.021.5304133
GATCGGA141600.021.4848441
TCTCGTA129300.021.33120241
CGGAAGA146050.021.0576864
CCAGTCA141200.020.91513626
Produced by FastQC (version 0.11.5)