FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28392931
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC324590.11432070891166538TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG76450.031.66032615
CGTCTGA77850.030.94649516
CTCGTAT61650.028.21007742
TCGGAAG89800.027.4574833
AGTCACT88550.027.0752828
ATCGGAA91950.026.6935522
TCGTATG65750.025.90520743
CACGTCT97450.025.1608914
CACACGT97850.025.05803712
ACTGACC95550.024.83240132
CAGTCAC103550.023.414127
CGTATGC70650.022.9622244
GTCACTG107100.022.44882629
AATCTCG99950.021.53878439
ACACGTC118950.021.0669713
CACTGAC115700.020.72179631
CGGAAGA126000.020.0164034
CCAGTCA122650.019.82248726
GATCGGA126250.019.6240221
GACCAAT123450.019.31526835
Produced by FastQC (version 0.11.5)