FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31371773
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC507230.16168356184395444TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG103950.034.1703915
ACTTAGG101950.033.9136632
CGTCTGA106950.033.2329316
CTCGTAT83950.031.11567942
TCGGAAG119050.030.424793
TCACTTA114550.030.24244930
TTAGGCA116050.029.99409734
TCGTATG84750.029.92205643
AGTCACT116150.029.8452828
ATCGGAA122100.029.573422
CACTTAG117750.029.4970431
GTCACTT118200.029.34670329
CACGTCT122250.029.2578214
CTTAGGC121600.028.69161433
CACACGT127050.028.34721412
CGTATGC91000.027.34797144
CAGTCAC133350.026.33332427
ACACGTC147900.024.57915113
TAGGCAT143900.024.10583335
CGGAAGA158900.023.2347834
Produced by FastQC (version 0.11.5)