FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30091842
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG62500.028.61370315
CGTCTGA63550.027.9639516
CTCGTAT55050.025.33923542
TCGTATG56700.023.45259943
ACCGATG78600.022.4538932
ATCGGAA80000.022.4410482
TCGGAAG81900.022.1399733
CACCGAT82450.021.26903331
CACGTCT87150.021.03671314
CACACGT88150.020.77251212
GATGTAT84950.020.49598735
CAGTCAC92600.019.37529427
CGTATGC67100.019.34839244
TATCTCG83250.018.88817439
GTATCTC93950.018.2694238
ACACGTC108050.017.38398713
AGTCACC104200.017.32637228
TCTCGTA86300.016.52651641
TGTATCT106150.016.16968737
GATCGGA114750.016.0603071
Produced by FastQC (version 0.11.5)