FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l01_n01_P1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l01_n01_P1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30548189
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC358910.11748977983604854TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG73450.030.19737615
CGTCTGA76000.029.06577916
CTCGTAT63650.028.13680842
TCGTATG69850.027.2842543
TCGGAAG90950.024.9085963
ATCGGAA91850.024.5667952
CGTATGC77250.024.46708344
CACGTCT97150.023.20116214
CACACGT99400.022.67594712
CAGTCAC103950.021.50440427
AGTCACA107550.020.67993528
GTCACAT108300.020.49515229
TCACGAT111800.019.57775735
GATCGGA122200.018.7823031
ACACGTC122300.018.7611113
TCTCGTA98200.018.60170741
ATCACGA125350.017.8966734
CCAGTCA125300.017.76794626
AGCACAC134700.017.33616310
GAGCACA135200.017.2556089
Produced by FastQC (version 0.11.5)