FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_ribopure_pooled_with_dnase.352000000be1a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_ribopure_pooled_with_dnase.352000000be1a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23705856
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA823410.3473445548644183No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC627350.26463925200591787No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA619990.2615345339143206No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG531950.22439603109037698No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC493270.20807938764160214No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG460910.19442875211930757No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG377850.15939099604755888No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA342880.14463936674549951No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC329610.1390415937732854No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG322690.13612248382846837No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA303340.12795994373710867No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG301630.12723860298484896No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG290530.12255621564561939No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC264220.11145769214155354No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG256590.10823907814170473No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC251970.10629019260051187No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT248420.10479267232535285No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT248340.10475892538957463No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG248220.10470830498590729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC257200.026.1043556
GAAACCC280850.024.1263073
GGTCGAA355150.023.7323975
AACCCAA305350.023.4796245
AGGTCGA366600.023.0446624
CGCCGAT171550.022.88657492-93
GCCGATC174800.022.54258292-93
CAACCCT293050.022.244759
CGATCTT184700.021.38110294-95
TCGAAGT396250.021.3776557
AGAAACC320500.021.3068622
AAACCCA350350.021.1162874
CCGATCT187250.021.0899394-95
GTCGAAG403000.020.9842476
CAGAAAC365500.020.7195661
ATACTCG195050.020.68642874-75
CCAACCC326400.020.612188
AACTCAC103350.020.3654065
TACTCGC199300.020.34090476-77
CCCAACC327000.020.2853817
Produced by FastQC (version 0.11.2)