FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_processing_control_2.352000000be353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_processing_control_2.352000000be353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64390
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG730.11337164155924832Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGA1300.091.397072
GACCGGA1050.090.5266342
ATGGGAG700.088.2634663
ACCGGAG700.088.2634663
TCGGGAG2100.086.00033
TCGGAGA1050.086.00034
CCGGAGA1300.084.0853044
ATCGGAG850.083.8702553
ACCGGAA750.082.379233
GAACCGG353.410605E-881.8564761
AACCGGA353.5235644E-881.473972
TCCGGAG650.080.429433
TCGGAGG650.080.429434
TTCGGAG600.079.21083
CGGGAGA2650.078.911894
GTTCGGA1150.078.522012
GGTCCGG551.8189894E-1278.135731
CGGAGAG2450.077.594255
GAGCGGC1350.077.390289
TCGGAAA800.077.230534
Produced by FastQC (version 0.11.2)