FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_processing_control_1.352000000be2b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_processing_control_1.352000000be2b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87272
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA3600.091.0576254
GACCGGA1500.088.682212
ATCGGAG1450.088.463783
TCGGAGA1600.086.108854
CGGAAAG2550.085.70135
TCGGGAG3050.084.1131063
GGACCGG2100.083.994621
GTTCGGA1250.083.6146552
TCCGGAA750.082.347773
GTCGGGA1850.082.176572
CCGGAAA1100.082.0598454
TTCGGAA700.081.4428563
GACGGGA1750.081.442852
GGATCGG3300.080.8991
ACGGGAA1300.080.398713
TCGGAGG1300.080.398714
ACCGGAA950.080.014023
TTGGGAG1850.079.608553
ATCGGGA2450.079.503732
TCCGGAG600.079.180553