FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_pcr_neg_control.352000000be3e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_pcr_neg_control.352000000be3e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51230
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGTA250.004995969656.994135
GAGCGGA300.003936261431.66340628-29
GACAGCG350.00836067127.14006234-35
ATCCTGA550.002861369421.58868644-45
GCGGACA650.007521743418.2673530-31
Produced by FastQC (version 0.11.2)