FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_neg_extraction_control_cb10-12_blood.352000000be104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_neg_extraction_control_cb10-12_blood.352000000be104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39020
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA156.5741385E-494.983293
GACCGGA353.2014214E-1094.983292
ATTCGGA156.5741385E-494.983292
TTCGGAA156.5741385E-494.983293
AAAGCGG650.094.983298
TCGGAGA650.087.676884
ATCGGGA650.087.676882
ATGGGAG600.087.068023
GGACCGG550.087.0195541
GGATGGG950.085.6455461
GTTCGGA500.085.484962
GTCCGGA500.085.484962
TTCGGAG500.085.484963
CCGGAGA950.084.985054
GAGGGGC1700.083.8087849
CCGGAAA409.222276E-1083.1103744
ATCGGAG353.504647E-881.414253
CGGGAGA1250.079.7859654
TCCGGAG600.079.152753
TCGGGAA600.079.152753
Produced by FastQC (version 0.11.2)