FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_3035_d3.352000000be049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_3035_d3.352000000be049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23357719
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA464750.19897062722605746No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC290770.12448561437013606No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA275400.11790534854880307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA210800.022.809415
AGGTCGA226650.021.5722984
CGCCGAT112500.019.84572692-93
ATACTCG119400.019.690874-75
TCGAAGT249400.019.5722567
GCCGATC115850.019.49737592-93
GTCGAAG252400.019.3019966
ACCCAAC181350.019.0615436
TACTCGC123950.018.7574376-77
CGCCATA131200.017.77765570-71
CGATCTT129750.017.39222394-95
CCGATCT130350.017.22047294-95
CCCGCGT134750.017.1497682-83
AACCCAA245000.016.9104275
GAAACCC212700.016.6656463
CTCGAAT47200.016.2776091
CCGCGTG144900.016.09596682-83
TCAGGTC390200.015.5038132
CGGCAAT34850.015.3363491
ACTCGCC154750.015.33105376-77
Produced by FastQC (version 0.11.2)