FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_3015_d3.352000000bdf80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_3015_d3.352000000bdf80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24025827
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA283140.11784818062662318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA158400.019.2567165
AGGTCGA176200.018.013814
ATACTCG84050.017.17940974-75
CTCGAAT49900.016.351841
TCGAAGT196900.016.1814487
CGCCATA89700.016.09951870-71
TACTCGC92750.015.77294576-77
GCCGATC89000.015.77218792-93
GTCGAAG200450.015.5632366
AATCGAC22700.015.4884595
ACCCAAC149900.015.236466
CGCCGAT91150.015.11352592-93
CGAAAAT45400.014.6093191
CGGCAAT39450.014.51460551
CGGAAAT49800.014.37251951
CGACAAT21050.014.2810281
TCGCTCG45200.013.8783992
CGCGAAT9300.013.8532531
CGCAAAT31600.013.59021951
AATCCAA177900.013.5404895
Produced by FastQC (version 0.11.2)