FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_3013_d8.352000000be005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_3013_d8.352000000be005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25905370
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA1211400.4676250522575049No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA1006330.38846385903772074No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC942520.36383190049013003No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG839840.3241953309294559No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG725310.2799844202186651No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC693190.2675854465695722No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC642800.24813388112194498No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG578380.22326645016071955No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA503510.19436510653968656No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC483930.18680682808236285No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG450290.17382110350093435No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG440080.16987983572517976No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG408150.1575542059426289No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA407510.15730715291848754No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC389410.15032018457949065No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT369370.14258433676106538No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG368770.14235272455093287No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG365250.14099393291815557No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG361970.1397277861694313No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC350940.1354699817064956No Hit
CCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT346070.1335900626009202No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT337820.1304053947115984No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA337680.1303513518625675No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG331440.12794258487718954No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT323410.12484284146491635No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC322360.12443752009718449No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA319760.12343386718661035No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT317300.12248425712506712No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT310570.11988634016808097No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT306280.11823031286563365No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC298380.11518075209888914No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG294050.113509283982433No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC290690.11221225560569104No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG290680.1122083954021888No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA287500.1109808506884866No Hit
GCCACACCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAA285970.11039023955264873No Hit
CGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTTG283520.10944448969460772No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC279850.1080277950092973No Hit
CTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGT279180.10776916137464936No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT279090.10773441954312947No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC269330.10396686092497423No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG264860.10224134995948715No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC264680.10217186629644741No Hit
CCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCC263280.10163143780613827No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA263050.10154265312558748No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC259450.1001529798647925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC338150.029.1796426
GAAACCC362200.027.2351743
AACCCAA394150.026.2665635
GGTCGAA488600.025.8469355
CGCCGAT228550.025.73102492-93
AGGTCGA494700.025.5968934
GCCGATC232850.025.58226492-93
AGAAACC400150.025.2131542
AAACCCA423700.024.963114
CAACCCT385650.024.9310769
CGATCTT240950.024.72259394-95
CCGATCT241350.024.50334494-95
CAGAAAC431000.024.3913291
CCCAACC410200.023.8922487
TCGAAGT529750.023.8515647
CCAACCC416250.023.6231468
GTCGAAG538300.023.5256926
ATACTCG262450.023.18307774-75
TACTCGC265150.022.84865876-77
CCCGCGT271050.022.22134282-83
Produced by FastQC (version 0.11.2)