FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_3012_d1.352000000bdf66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_3012_d1.352000000bdf66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25355337
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA1336370.5270566902739254No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC1074910.423938360590514No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA1034550.40802060725913447No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG868820.3426576424521591No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC797790.31464381640835615No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG726900.2866852055644143No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG704120.27770090375844736No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA641570.2530315412490869No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG585060.23074432021944727No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC567140.22367677463722924No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG553040.21811581522264917No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC513700.2026003440616861No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT509730.2010345987513398No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA496970.19600212767828723No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG490150.19331235865648325No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT489930.19322559191384442No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG485860.1916204071750259No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG472670.18641834655954287No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC465120.18344066970989184No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT452140.17832143189420044No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA444110.17515444578788283No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT441010.1739318235052447No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT438910.17310359550732848No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG436160.17201901122434302No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG435140.1716167290539266No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT435080.1715930653968433No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG433120.17082005259878819No Hit
CGCCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACG417660.1647227169569862No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC406490.1603173327966416No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC395760.15608548212157464No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA394930.1557581348652554No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC388530.15323401144303467No Hit
CCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCC377080.1487181968829679No Hit
GTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAG373430.1472786577437326No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC369080.14556304260519196No Hit
GTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGAC368340.1452711908344977No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC365920.14431675666547047No Hit
TGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAAC359650.1418439045002636No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC356850.14073960050304202No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG354560.13983643759102868No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC353080.13925273404964011No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA352890.13917779913554296No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG344130.1357229052013783No Hit
AAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCAT342920.13524568811686472No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC333230.1314240074979086No Hit
CTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAA328280.1294717557885348No Hit
CTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTG327260.12906947361811835No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC326970.128955099275549No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA326250.12867113539054914No Hit
CTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGT314410.12400150705944078No Hit
GAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGT313910.12380430991707977No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC311260.12275916506256652No Hit
AAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCA307180.1211500363809008No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC303100.11954090769923507No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA302700.11938314998534628No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC299120.11797121844604155No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT296110.11678409164902837No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC294520.1161570047363204No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC293470.11574289073736231No Hit
CCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT292590.11539582376680697No Hit
CAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCG292190.11523806605291817No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT292030.11517496296736265No Hit
GTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAA287730.11347906754305809No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC286550.11301368228708614No Hit
CCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAA280060.11045406337924044No Hit
CCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGG274940.10843476464146384No Hit
CACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAG274260.1081665765278529No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG270530.10669548584583988No Hit
CAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGCAGTTTGT266440.10508241322132693No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT265870.1048576084790354No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA265790.10482605693625764No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG261030.10294874014098096No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC260700.1028185900270227No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC259240.1022427743713286No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC258540.10196669837202323No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG256570.10118974163112089No Hit
GCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCA256450.10114241431695425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC389650.028.7347226
AACTCAC122750.028.564855
ACTCACC126900.027.614216
GAAACCC403750.027.5977573
AACCCAA427500.027.0397765
AAACCCA455650.025.9757334
AGAAACC433950.025.9754522
CAGAAAC453800.025.7730831
CAACCCT429700.025.6897249
CCCAACC443950.025.070417
CCAACCC447600.025.0655488
CGCCGAT269050.024.89481792-93
GCCGATC273350.024.88552392-93
GGTCGAA598300.024.2281535
CGATCTT281300.024.08379694-95
CCGATCT280600.023.97348894-95
AGGTCGA608500.023.8392014
GAACTCA157050.023.144564
AGAACTC149250.022.922483
TCGAAGT635200.022.7846497
Produced by FastQC (version 0.11.2)