FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n02_3004_d3.352000000bdfaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n02_3004_d3.352000000bdfaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25484506
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA317120.12443639284198799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA177450.018.661965
ATACTCG86850.018.2114274-75
AGGTCGA196650.017.3002034
CGCCGAT91350.016.74446792-93
GCCGATC93600.016.62109292-93
TACTCGC97200.016.2723276-77
ACCCAAC169450.015.69232756
CGCCATA101400.015.60037470-71
TCGAAGT222150.015.5605877
CTCGAAT51100.015.4077771
GTCGAAG221750.015.18180756
CGACGAT18750.014.7605891
CGACAAT24650.014.5184851
AACCCAA234800.014.4072935
CGATCTT110700.014.18626394-95
TCGAATC30200.014.1620392
GAAACCC206150.013.714513
CGCCGAC133850.013.6895691
CCCGCGT115250.013.68377382-83
AATCCAA182100.013.5934065
Produced by FastQC (version 0.11.2)