FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_ribopure_pooled_with_dnase.351000000be1a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_ribopure_pooled_with_dnase.351000000be1a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23705856
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA931650.3930041589723653No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA636040.2683050129048282No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC615680.25971641774926835No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG531450.22418511274176303No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC484890.20454439611883238No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG463960.19571535404585266No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG374190.1578470737357048No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA329580.13902893867236854No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG311100.1312333965075971No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC303380.1279768172049978No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA302190.1274748315352966No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG286100.12068747907689982No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG270470.11409417149922786No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG268780.11338126748091273No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC253730.10703262518763296No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT252010.10630706606840098No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG250800.10579664366475523No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG249790.10537058860055507No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT245020.10335842755477803No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC244870.10329515205019385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC256300.026.1080426
GAAACCC280500.024.0252973
CGCCGAT191050.023.42965792-93
AACCCAA307050.023.416865
GCCGATC194450.023.38634992-93
GGTCGAA405400.023.1129465
AGGTCGA418200.022.4058024
CAACCCT292400.022.088879
CGATCTT206800.021.97775894-95
AAACCCA348500.021.7221574
CCGATCT209300.021.54506194-95
AGAAACC319450.021.0810682
ATACTCG219800.020.87629574-75
TCGAAGT453500.020.8081047
TACTCGC219850.020.60105776-77
GTCGAAG456950.020.5574656
CCCAACC323550.020.3879137
CCAACCC324050.020.3564558
CAGAAAC367850.019.8386761
CCCGCGT230600.019.74997182-83
Produced by FastQC (version 0.11.2)