FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_processing_control_2.351000000be356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_processing_control_2.351000000be356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64390
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT3238350.29197080291971TruSeq Adapter, Index 21 (97% over 40bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT5710.8867836620593259TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTAT2970.46125174716570894TruSeq Adapter, Index 21 (97% over 40bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT2790.43329709582233267TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGCAT2200.34166796086348816TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT2020.3137133095201118TruSeq Adapter, Index 21 (97% over 40bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1940.3012890200341668TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTCT1300.20189470414660662TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAACCTCGTAT1250.19412952321789098TruSeq Adapter, Index 21 (97% over 40bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT770.11958378630222083TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGCATCTCGTAT720.1118186053735052TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGACTCTCGTAT660.10250038825904642TruSeq Adapter, Index 21 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA750.089.071031
ACCGGAA750.088.6557852
GAGCACA39350.088.229339
GAAGAGC39600.087.552396
AGAGCAC39700.087.4514858
AAGAGCA39600.087.432467
CGGAAGA39950.087.023124
GGAAGAG39850.087.003135
GATCGGA39200.086.668981
TCGGAAG39300.086.2871253
ATCGGAA39350.086.177482
CCGGAAG800.083.114813
AGGTGCA200.002066168271.2412644
TCAGGTG301.2927363E-463.3255652
CAGGTGC250.005002246256.9930083
CGTGAAG250.005002246256.9930089
AGCGGAA352.7698398E-454.2790532
GGATGCC205.373504E-447.49417546-47
GCGGAAG405.353355E-447.4941753
TATCGTA452.237357E-1047.4941742-43
Produced by FastQC (version 0.11.2)