FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_processing_control_1.351000000be2b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_processing_control_1.351000000be2b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87272
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT4776354.728893574113116TruSeq Adapter, Index 20 (97% over 44bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT8680.9945916215968467TruSeq Adapter, Index 20 (97% over 41bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT3870.44344119534329457TruSeq Adapter, Index 20 (97% over 41bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2790.31969016408470075TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTACCTCGTAT1670.19135576129801082TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGCAT1450.16614721789348244TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1250.14323036025300212TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTAT1100.12604271702264186TruSeq Adapter, Index 20 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTCTCTCGTAT1100.12604271702264186TruSeq Adapter, Index 20 (97% over 44bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1060.12145934549454579TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTCT1020.11687597396644973TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC55000.090.806318
GAGCACA55300.090.571249
CGGAAGA55250.090.481364
GAAGAGC55200.090.477316
ATCGGAA53350.090.411222
TCGGAAG53300.090.406953
GATCGGA53600.090.321951
GGAAGAG55500.090.0737845
AAGAGCA55500.089.90277
ACCGGAA900.089.67462
GACCGGA950.085.101191
CCGGAAG1000.080.707133
AGCGGAA700.074.603232
ACGGAAG650.073.038133
GAACGGA750.069.7495961
GCGGAAG750.069.629683
AACGGAA900.058.024742
GAGCGGA950.055.0654751
TATCGTA205.3592364E-447.52926342-43
CCTCGTA252.8068223E-547.5292642-43
Produced by FastQC (version 0.11.2)