FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_pcr_neg_control.351000000be3e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_pcr_neg_control.351000000be3e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51230
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1850.059.052844
GCCCGTC250.00499439556.998836
GCCGTCT2000.054.623886
TATGCCG2050.053.2915883
CGTATGC2150.050.9123461
GTATGCC2250.048.5545582
TGCCGTC2250.048.5545585
CGTCTTC2500.045.5990648
GTCTTCT2550.042.842269
CCGTCTT2850.038.3325427
CTTGAAA2000.027.3119416-17
TTGAAAA2050.026.64579418-19
GCTTGAA2150.025.40645416-17
TGCTTGA2200.024.82903514-15
GAAAAAA2200.024.82903520-21
CTGCTTG2300.024.78209914-15
TCTTCTG2350.023.24420410-11
TGAAAAA2600.021.92262618-19
TTCTGCT2650.021.50899112-13
TCTGCTT2600.021.00918412-13
Produced by FastQC (version 0.11.2)