FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_neg_extraction_control_cb10-12_blood.351000000be107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_neg_extraction_control_cb10-12_blood.351000000be107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39020
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1801346.16350589441312TruSeq Adapter, Index 9 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3040.779087647360328TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC2700.6919528446950282TruSeq Adapter, Index 9 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1410.36135315222962583TruSeq Adapter, Index 9 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC990.2537160430548437TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC830.21271143003587903TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGCATGC660.16914402870322912TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC490.1255766273705792TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC470.12045105074320861TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC460.11788826242952331TruSeq Adapter, Index 9 (98% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC440.11276268580215273TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTCTGC430.11019989748846745TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA21450.089.4590454
GAAGAGC21300.089.197066
ATCGGAA21100.089.1421052
TCGGAAG20950.089.10023
GGAAGAG21450.089.0161745
GATCGGA21100.088.9228061
GAGCACA21300.088.7510769
AGAGCAC21250.088.736398
AAGAGCA21500.087.925487
ACCGGAA353.5019184E-881.424182
CCGGAAG353.5019184E-881.424183
CGGAAAG255.213071E-575.99594
GCGGAAG255.213071E-575.99593
GACCGGA408.526513E-871.61341
GAGTACA200.0020594871.2461559
AGAGTAC200.0020594871.2461558
GAGCGGA353.255369E-668.203241
GGAAGAC250.004986139556.9969255
GAAAGAG352.755332E-454.2827846
AGCGGAA352.755332E-454.2827842
Produced by FastQC (version 0.11.2)