FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_3035_d3.351000000be04c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_3035_d3.351000000be04c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23357719
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA498970.21362103037544036No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC283010.12116337215975584No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA279050.11946800113487109No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG233590.10000548426839112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA227700.022.463025
AGGTCGA244700.021.1743014
CGCCGAT119400.020.72350992-93
TCGAAGT265050.019.5305377
GCCGATC127200.019.45272692-93
ATACTCG127400.019.4067774-75
GTCGAAG271450.019.0700636
TACTCGC131400.018.86988376-77
CGATCTT138050.017.71733594-95
CCCGCGT140400.017.6491682-83
CCGATCT141150.017.59739594-95
CGCCATA141950.017.45109770-71
ACCCAAC187250.017.2719256
CCGCGTG151700.016.55374382-83
CGCGTGG153100.016.23167484-85
GAAACCC210900.016.2134743
ACTCGCC154600.016.19180176-77
AACCCAA251150.015.8840645
CGCGAAT6950.015.7647191
TCGCCCG162400.015.444489578-79
Produced by FastQC (version 0.11.2)