FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_3031_d1.351000000bdfc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_3031_d1.351000000bdfc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33461527
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA2990760.8937906509765678No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA1934900.5782461750774255No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC1717030.5131355780625313No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG1589650.4750679788163881No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC1488800.44492888803311337No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG1320780.39471599727053697No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG1210530.3617677101227329No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA1016750.3038564259186379No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC958060.2863168796809542No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG926020.2767417039873883No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC913070.2728715877192335No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG876110.2618260666944458No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA872930.2608757215413391No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT856030.25582514509872784No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG818480.2446033021744644No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG818430.24458835964061054No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG810700.24227824390680078No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA789330.23589180493765274No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG782590.23387755137414976No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG769670.23001640062630732No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT762480.22786766425811947No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG752460.22487318047380206No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT748580.223713639846741No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT743350.22215065080562524No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT740840.2214005356061605No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC724700.21657708567812817No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA719450.21500811962347086No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC704810.21063294571105495No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC696880.2082630598418297No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT684170.20446466773617356No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA673920.20140144829612827No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC657460.19648236615143116No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT656110.19607891773737643No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC644610.19264213495098417No Hit
GTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGAC637630.1905561572249826No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC627100.18740925959535557No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC624980.1867756961599511No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG601780.17984236045175103No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC601660.17980649837050175No Hit
CGCCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACG591950.17690465829607838No Hit
GTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAA580620.17351868012478927No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC579770.17326465704927332No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT575190.17189592094825798No Hit
GTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAG572940.17122350692483343No Hit
CTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAA570120.17038074801547462No Hit
CTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTG562730.16817224151187124No Hit
CCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCC560820.16760143671865302No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC553290.1653510911202588No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA552100.16499545881453645No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC552020.16497155076037026No Hit
CTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGT548320.16386580325518318No Hit
TGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAAC545970.16316350416405084No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA545750.16309775701509377No Hit
AAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCAT544460.16271223964166368No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG542290.1620637336724053No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC539470.16122097476304653No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC528240.15786488165946522No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT526470.15733591596103788No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG519870.1553635014923258No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG512740.1532326961647626No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC510770.15264396033091973No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC508750.15204028196322303No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC505100.15094947699188982No Hit
GAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGT501560.14989154559503517No Hit
CGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAG501440.14985568351378584No Hit
CCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGG494600.14781154488257514No Hit
AAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCA494340.14773384370653497No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA467950.13984717433845742No Hit
CAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCG462660.13826625425671699No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC461490.13791659896453617No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC460250.13754602412496No Hit
CACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAG456850.1365299318228962No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG449990.13447981617814392No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC447620.13377154007347006No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG439270.1312761369198722No Hit
CAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGCAGTTTGT437830.13084579194488044No Hit
GTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGAC435420.13012556181312346No Hit
CCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT428960.12819498643920224No Hit
CCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAA426960.12759728508504709No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG426290.1273970551314051No Hit
CTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCA424120.12674854916214673No Hit
GTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTC422350.1262195834637194No Hit
CTCTGAGCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTC419240.1252901578580081No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT416760.12454900817885567No Hit
GCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAGG412380.12324004221325584No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA411580.12300096167159377No Hit
ATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTG410020.12253475461535272No Hit
GTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTC409300.12231958212785686No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA405090.12106142077736022No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA402150.12018279978675211No Hit
GCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCA385900.1153264762842413No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC385120.11509337275612079No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA382230.11422969429936655No Hit
CTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGA378200.11302532607074388No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA369850.11052992291714601No Hit
CTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAGAAGG363250.10855750844843393No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA362280.10826762329166867No Hit
CGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGG362170.10823474971719013No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC360360.1076938299916797No Hit
CGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGA358770.10721865741512633No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT356100.10642072610732917No Hit
CACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCC354380.10590670294275573No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG353720.1057094614958845No Hit
GTCGCTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGG348110.10403290919747923No Hit
CCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGG346140.1034441733636364No Hit
GCCACACCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAA345790.10333957562665924No Hit
GTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAA345530.10326187445061906No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT344820.10304969046989398No Hit
CTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGG343950.10278969038083646No Hit
CGCCGACAAGACCAACGTCAAGTCCACCTGGGGCAAGATCGGCGGCCACG342630.10239520748709406No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT341360.10201566712720553No Hit
CAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGCC339950.10159428767252614No Hit
GCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTG339160.10135819563763483No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC339140.10135221862409328No Hit
CTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAG338010.10101451735899561No Hit
TGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTC337670.10091290812878922No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT336120.10044968957931895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC161100.035.75925
ACTCACC170150.033.2152526
ACCCAAC555400.030.9973566
GAACTCA193750.030.1992444
GAAACCC571500.029.9584063
AACCCAA593150.029.6250925
CAGAAAC607400.028.9625841
AGAACTC192350.028.9624613
AGAAACC596750.028.74652
AAACCCA621250.028.7287984
CCCAACC617150.027.8189097
CAACCCT614400.027.7888269
CCAACCC627350.027.502878
CGCCGAT537600.026.81273592-93
GCCGATC543400.026.80186392-93
CGATCTT551800.026.35917794-95
CCGATCT554750.025.99641494-95
GGTCGAA1166250.025.8545025
AGGTCGA1177800.025.5407454
TCGAAGT1234550.024.466457
Produced by FastQC (version 0.11.2)