FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_3015_d3.351000000bdf83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_3015_d3.351000000bdf83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24025827
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA301100.1253234696145943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA175650.018.6558345
ATACTCG89400.017.82628874-75
AGGTCGA196550.017.155474
CGACAAT21100.016.9316271
GCCGATC93300.016.92570592-93
CGCCATA97300.016.33014770-71
TCGAAGT211500.016.2570577
TACTCGC99350.015.94489676-77
CGCCGAT97800.015.83126192-93
GTCGAAG213450.015.5968046
CTCGAAT47900.015.4140261
CGACGAT17750.015.2966511
TATGCCG53450.014.7496373
CGATCTT109300.014.70847494-95
CGTATGC55150.014.5105441
CGGCAAT40750.014.4948581
CAATCGA17100.014.4419074
CTCGGAT61250.014.3097361
CGAAAAT45750.014.1601451
ACCCAAC152000.014.1536466
Produced by FastQC (version 0.11.2)