FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_3012_d1.351000000bdf69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_3012_d1.351000000bdf69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25355337
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA1487720.5867482652665985No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA1054260.41579411861100485No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC1019010.4018917200745547No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG883100.3482895928379891No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC776100.30608940437273624No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG725030.2859476882519842No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG704270.2777600629011557No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA641630.25305520490617023No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG576930.22753789468465754No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG556580.219511970990565No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC541800.213682823462374No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG528240.20833483696154387No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA521170.20554646936855936No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG505490.19936236698411858No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC498020.1964162416772453No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT495330.19535532105134315No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC489730.1931467130569No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG485260.19138377060419273No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG483970.19087500197690135No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT465950.1837680169662111No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT451800.17818733783739493No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT446200.1759787298429518No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA431320.1701101428862886No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT428580.16902950254615035No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT421280.1661504242676798No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG417270.16456890318594464No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC410030.16171348856455744No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC407350.1606565118815025No Hit
GTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGAC402250.15864510102942037No Hit
TGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAAC400150.15781687303150418No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG397740.15686638280532417No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC389220.15350614349949282No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA382010.15066256070664727No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC381700.15054029847838346No Hit
CGCCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACG379660.14973573413755062No Hit
CTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAA379170.14954248093803682No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC378910.1494399384240091No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA374050.14752318220026026No Hit
CCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCC367760.14504244214935894No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC365670.14421815809428998No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG361570.14260114152692982No Hit
GTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAG359750.1418833439287358No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC359650.1418439045002636No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG359090.14162304370081927No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC358950.1415678285009582No Hit
CTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTG353950.13959585707734826No Hit
GTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAA338120.13335259555019915No Hit
AAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCAT335910.13248098418096355No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC335830.13244943263818582No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT332080.13097045407047833No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA329890.1301067305869372No Hit
GAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGT329260.12985826218756233No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC324600.12802038482075787No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC323440.12756288745048033No Hit
CCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGG321860.1269397444806196No Hit
AAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCA313320.12357161728909381No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC313200.12352428997492718No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA313030.12345724294652444No Hit
CTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGT310180.12233321923506676No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC308970.12185600215055316No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT302680.11937526209965185No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC290100.11441378199784921No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG281000.1108247940068791No Hit
CGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAG279550.11025292229403222No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA278870.10998473418042126No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC278380.1097914809809075No Hit
CCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT275540.10867140121229706No Hit
CCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAA275020.1084663161842416No Hit
GCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAGG274840.10839532521299165No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT273010.10767358367195042No Hit
ATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTG272820.10759864875785324No Hit
CACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAG268280.10580809870521539No Hit
CAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGCAGTTTGT267690.10557540607722943No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC266960.10528749824938238No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA263680.10399388499549424No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG262710.10361132253931392No Hit
CAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCG261880.10328397528299466No Hit
TGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTC260120.10258984134188397No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC257180.10143032214480131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC121650.029.8257775
ACTCACC127400.027.920486
ACCCAAC383750.027.0280516
AACCCAA415850.025.7182945
GAAACCC403850.025.6596263
CGCCGAT286750.025.075892-93
GCCGATC294900.024.69683892-93
AGAAACC430150.024.421982
CAACCCT424750.024.2625739
CAGAAAC450450.024.2158491
AAACCCA453600.024.1434974
GAACTCA154300.023.9457054
CCCAACC432850.023.929237
CGATCTT306000.023.87036794-95
CCGATCT302350.023.82866994-95
CCAACCC438750.023.6940428
AGAACTC150450.023.0118223
GGTCGAA658000.022.8288355
AGGTCGA665950.022.5208324
TCGAAGT701950.021.4603847
Produced by FastQC (version 0.11.2)