FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l08n01_3004_d3.351000000bdfad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l08n01_3004_d3.351000000bdfad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25484506
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA357180.14015574796701966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA202000.019.419795
ATACTCG99100.018.9092974-75
CGCCGAT101200.018.32636692-93
GCCGATC102750.018.28102592-93
AGGTCGA218950.018.1552184
TACTCGC107800.017.29483476-77
CGCCATA109300.017.03597570-71
ACCCAAC166900.016.5608446
TCGAAGT245400.016.2562587
GTCGAAG246650.016.0583446
CGATCTT119000.015.76450894-95
CCGATCT125250.015.11057394-95
AACCCAA233400.014.8538095
CCCGCGT129050.014.52485982-83
CTCGGAT71100.014.4716061
GAAACCC202900.014.2546953
AATCCAA176800.014.1829815
CTCGAAT51400.014.1795011
AATCGAG34200.014.1641295
CCGAAAT40500.013.9966641
Produced by FastQC (version 0.11.2)