FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3035_d-2.352000000be5d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3035_d-2.352000000be5d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17791222
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA942370.5296825591856479No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC868800.4883307060077155No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA804280.45206563101736347No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG742360.41726195086543244No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC673720.3786811271311212No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG567420.31893256123722136No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA498060.2799470435476551No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG485670.2729829350676418No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC474490.266698937262432No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG464440.26105008413699743No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG445370.25033131507211814No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG440310.247487215886576No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA432120.24288382214555018No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT422900.23770149121853462No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG402210.22607216075433154No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC391040.2197937836985003No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA375330.21096358642481106No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG372130.20916494662367768No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG348190.1957088726114485No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT345790.19435989276059845No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT334720.18813772319855263No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC327030.18381536692645395No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG314530.17678943020327664No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC312840.17583952355830307No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT311530.17510320538971408No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG307000.17255700592123466No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC298020.16750957297930405No Hit
CCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT292340.1643169873322923No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC287300.1614841296455072No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC285440.16043867026109843No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT283140.1591458979040338No Hit
CGCCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACG282730.15891544717951359No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG280820.1578418840482121No Hit
CCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCC280790.15782502180007646No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC278030.1562736949715989No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC276420.15536875432165367No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA275480.15484040388007075No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT274460.15426708744345946No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC272500.15316542056526528No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG267340.1502651138859377No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC266730.14992224817384664No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC265190.14905665276955118No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG264880.14888240953881637No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC260530.1464373835591507No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC260230.14626876107779443No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG257360.14465560600615293No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC256920.1444082930334971No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA252390.14186209356501764No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT249630.14031076673654008No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA247560.13914727161518192No Hit
GTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAG247460.1390910641213965No Hit
GCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAGG233540.13126698098646625No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC229280.12887254175120744No Hit
GTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGAC229090.12876574751301514No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT228510.1284397440490597No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC226990.12758539014352133No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA225070.12650620626284131No Hit
CAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGCAGTTTGT224110.12596661432250128No Hit
CGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTTG221770.12465135896792251No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC219670.1234710015984287No Hit
AAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCAT216780.1218466050280301No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA215080.12089107763367798No Hit
GCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTG213840.12019410471073881No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC210980.11858657038847584No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC209720.11787835596677956No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC209610.1178165277236156No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC208630.11726569428451852No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA208490.11718700379321893No Hit
CTCTGAGCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTC207670.11672610234417849No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG204320.11484315130236697No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG204250.11480380605671718No Hit
CAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCG200940.11294333801241983No Hit
CTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGT199280.11201029361558189No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT198830.1117573598935475No Hit
CACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAG198380.11150442617151313No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG197830.11119528495569331No Hit
GAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGT197670.11110535296563666No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA197620.11107724921874393No Hit
TGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTC194250.10918305667817535No Hit
CCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAA194220.1091661944300397No Hit
CGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAG193680.10886267396359844No Hit
GCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCA193410.10871091373037783No Hit
TGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAAC192160.10800832005806009No Hit
TGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGC190840.10726638114009258No Hit
GTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTC190610.1071371039043861No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC190130.10686730793421609No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA189510.10651882147274651No Hit
CTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCA189010.10623778400381942No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG186020.1045571799396354No Hit
GCCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGC183400.10308454360245743No Hit
AAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCA181780.10217398220313365No Hit
CGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGA180620.1015219752752228No Hit
GCCACACCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAA180420.10140956028765198No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT179770.10104421157804676No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC178460.10030789340945777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC282650.032.3384136
GAAACCC289950.031.4924873
AACCCAA303800.030.676945
CAGAAAC310650.030.1718041
AGAAACC306100.030.0795972
AAACCCA322800.029.596934
CAACCCT316900.028.6909129
CCCAACC325100.028.1594757
CCAACCC325750.028.1299828
AACTCAC69150.026.6526495
ACTCACC72650.025.032266
GCCGATC196100.024.41679692-93
CGCCGAT193700.024.2656592-93
CGATCTT202050.023.695794-95
CCGATCT202350.023.25894494-95
GGTCGAA477900.021.5388585
AGGTCGA485600.021.2397424
ATACTCG238700.020.99442374-75
GAACTCA88550.020.9239754
CCCGCGT236050.020.70763882-83
Produced by FastQC (version 0.11.2)