FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3033_d8.352000000be544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3033_d8.352000000be544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18870575
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA471020.24960553666223737No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC468440.24823832872077292No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA444160.2353717361553636No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG400080.2120126175275528No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC342810.18166378078039488No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG318200.16862231278061215No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG273040.14469087454939766No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG244490.1295614998483088No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA243150.12885139959964123No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG237780.12600569934938388No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC235520.12480806758670576No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA226120.11982676733485864No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG220050.1166101191935063No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT215710.1143102422687173No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC215560.11423075343491122No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG208400.1104364864345681No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA193200.10238161794221957No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG192020.10175630578294514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC190800.025.0607936
GAAACCC211900.022.712113
AACCCAA227700.022.5303655
CGCCGAT109500.021.29951192-93
CAACCCT221400.021.2092029
GCCGATC114000.020.91708692-93
AAACCCA261100.020.7244554
ATACTCG122150.020.29967774-75
AGAAACC245050.019.911552
CAGAAAC273150.019.878841
TACTCGC123750.019.80535176-77
CGATCTT122150.019.72275494-95
GGTCGAA259550.019.5642935
CCGATCT120500.019.51679694-95
CCAACCC247300.019.410328
CCCAACC247250.019.2045947
AGGTCGA268550.019.0527154
CGCCGAC160950.018.6010361
CGCCATA138150.017.95021870-71
CCCGCGT135750.017.88145882-83
Produced by FastQC (version 0.11.2)